MCQs on Sterilization, Culture Media & Techniques for HPRCA Assistant Staff Nurse Exam 2026
Sterilization, culture media, and microbiological techniques are core subjects for candidates preparing for the HPRCA Assistant Staff Nurse Examination 2026. These topics play a vital role in understanding infection control, prevention of hospital-acquired infections, and safe nursing practices. A staff nurse must possess sound knowledge of sterilization methods, disinfectants, antiseptics, types of culture media, staining techniques, and laboratory diagnostic tests to ensure patient safety and effective healthcare delivery.
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In competitive nursing examinations conducted by HPRCA, questions from microbiology are frequently asked in the form of Multiple Choice Questions (MCQs). These MCQs are designed to test both conceptual clarity and practical understanding. Topics such as pasteurization, lyophilization, thermal death time, toxin detection tests, and classification of culture media carry significant exam weightage.
This MCQ set has been prepared strictly according to the latest HPRCA Assistant Staff Nurse syllabus and exam pattern for 2026. Each question is framed to enhance critical thinking and quick decision-making skills. Practicing these MCQs regularly will help candidates improve accuracy, boost confidence, and perform better in the final examination.
MCQs on Sterilization, Culture Media and Techniques
Q1. The medium used in membrane filter technique was
(A) EMB agar
(B) EMR-Vp medium
(C) Lactose broth
(D) Endo agar
Answer: (D) Endo agar
Q2. Lysol is a
(A) Sterilent
(B) Disinfectant
(C) Antiseptic
(D) Antifungal agent
Answer: (B) Disinfectant
Q3. Which of the following is a neutral stain?
(A) Picric acid
(B) Giemsa
(C) Neutral red
(D) Malachite green
Answer: (C) Neutral red
Q4. Peptone water medium is an example for
(A) Synthetic medium
(B) Semisynthetic medium
(C) Differential medium
(D) None of these
Answer: (B) Semisynthetic medium
Q5. The method in which the cells are frozen dehydrated is called
(A) Pasteurization
(B) Dessication
(C) Disinfection
(D) Lypophilization
Answer: (D) Lypophilization
Q6. The technique used to avoid all microorganisms is accomplished by
(A) Sterilization
(B) Disinfection
(C) Surgical sterilization
(D) Disinfection Sterilization
Answer: (A) Sterilization
Q7. Thermal death time is
(A) Time required to kill all cells at a given temperature
(B) Temperature that kills all cells in a given time
(C) Time and temperature needed to kill all cells
(D) All of the above
Answer: (B) Temperature that kills all cells in a given time
Q8. A culture medium the exact composition of which is not known was called as
(A) Simple
(B) Complex
(C) Defined
(D) Natural
Answer: (A) Simple
Q9. Elek’s gel diffusion test is used for the detection of
(A) Tetani toxin
(B) Cholera toxin
(C) Diphtheria toxin
(D) Toxoid
Answer: (C) Diphtheria toxin
Q10. Temperature required for pasteurization is
(A) Above 150°C
(B) Below 100°C
(C) 110°C
(D) None of these
Answer: (B) Below 100°C
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